mouse cd8 t cell enrichment columns  (R&D Systems)

Journal:

Article Title: Primary Role for CD4 + T Lymphocytes in Recovery from Oropharyngeal Candidiasis

doi:

Figure Lengend Snippet: Time course of C. albicans clearance in BALB/c nu/nu mice reconstituted with 2.5 × 106 naive CD4+ or CD8+ T cells. The positive control group received 3 × 107 naive lymphocytes, while the negative control group received no cells. Bars represent scores (mean ± SEM) for a minimum of 10 mice/group. Each experiment was repeated twice. Comparison was made between animals that received either CD4+ or CD8+ T cells and negative controls. ∗, significantly different at P < 0.001 for CD4 group; •, significantly different at P < 0.05 for CD8 group. Positive control group was significantly different from negative control group at P < 0.001 from days 21 to 70.

Article Snippet: T-cell enrichment was carried out using commercially available CD4 + and CD8 + mouse T-cell enrichment columns (R&D, Minneapolis, Minn.), according to the manufacturer's instructions.

Techniques: Positive Control, Negative Control

Journal:

Article Title: Primary Role for CD4 + T Lymphocytes in Recovery from Oropharyngeal Candidiasis

doi:

Figure Lengend Snippet: Representative agarose gel stained with ethidium bromide showing CD4 and CD8 gene products in BALB/c nu/nu mice following lymphocyte reconstitution. RT-PCR was performed on BALB/c tongue and oral mucosa. Lane 1 shows size markers, while lane 2 shows the cDNA negative control for each gel. CD4 is not present in control nude mice (lane 3), whereas CD8 is present at all times (lanes 4, 6, and 8). CD4 is present in oral mucosa on day 4 (lane 5) and day 8 (lane 7) following lymphocyte transfer, but is not seen in the tongue until day 21 (data not shown).

Article Snippet: T-cell enrichment was carried out using commercially available CD4 + and CD8 + mouse T-cell enrichment columns (R&D, Minneapolis, Minn.), according to the manufacturer's instructions.

Techniques: Agarose Gel Electrophoresis, Staining, Reverse Transcription Polymerase Chain Reaction, Negative Control